A method for rapidly detecting hepatitis A virus by using near-infrared fluorescent immunoassay was established. A monoclonal antibody against hepatitis A virus was labeled with a near-infrared fluorescent dye. Polyclonal antibody and goat anti-mouse IgG polyclonal antibody were sprayed on a nitrocellulose membrane . Then those were used as test line and control line to develop NIR immunoassay strips for detecting hepatitis A virus. Studies have shown that NIR immunoassay has good specificity and high sensitivity for detecting hepatitis A virus. The minimum detection limit is 1 μg/mL and no cross reaction with EV71, adenovirus, rotavirus and norovirus. Through the effect evaluation test, it was found that NIR immunoassay method has the shortest detection time compared with the traditional and colloidal gold method. The detection limit is lower than the colloidal gold. Thus, NIR immunoassay can be used for detecting hepatitis A virus and provide reliable technical support for food safety supervision.
WAN Xiaonan
,
CHANG Xiaohui
,
QI Wei
,
GAO Xin
,
QIAO Bin
,
YANG Xiangying
,
LI Xiaolin
,
ZHANG Huiyuan
,
SHI Song
,
ZHANG Jie
,
ZHOU Xicheng
. Rapid detection method of foodborne hepatitis A virus based on near-infrared immunochromatography[J]. Food and Fermentation Industries, 2020
, 46(7)
: 213
-217
.
DOI: 10.13995/j.cnki.11-1802/ts.021114
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