In order to achieve high sensitivity and rapid detection of Salmonella in chicken, a new method based on fluorescence labeling DNA-magnetic graphene oxide(GO) magnetic separation technology was established. The method was used to optimize the detection conditions of Salmonella target DNA and to detect Salmonella in artificially contaminated chicken. The results showed that the optimum detection conditions for Salmonella target DNA were as follows: Fe3O4/GO 3.2×10-4 g/mL, reaction time of Fe3O4/GO with Salmonella capture probe 60 min, 37 ℃, hybridization time of DNA probe 120 min and enrichment multiple 5 times. There was a good linear relationship between the target DNA concentration and fluorescence intensity within the range of 0.005-1 pmol/L, and the lower limit of detection was 5 fmol/L (S/N=3). Under optimal conditions, the minimum detection limit for Salmonella contaminated chicken samples was 102 CFU/mL (S/N=3). This method only needed 5 h in the whole detection process, with simple operation, high sensitivity, strong specificity and accurate and reliable results, which could realize the rapid detection of Salmonella. This study can provide a new idea for the detection of Salmonella and other pathogenic bacteria.
MENG Yuanyuan
,
LIU Lili
,
YANG Xiaopan
,
DAI Xiaoning
,
CHEN Ke
. Rapid detection of Salmonella in chicken meat by fluorescent labeled DNA-magnetic graphene oxide magnetic separation technology[J]. Food and Fermentation Industries, 2020
, 46(12)
: 251
-257
.
DOI: 10.13995/j.cnki.11-1802/ts.022085
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