The nutritional value and protein composition of Chinese soft-shelled turtle were investigated, followed by structural and functional analysis of protein extracted by alkali solution and acid precipitation. The results showed that the protein content of Chinese soft-shelled turtle was 18.61 g/100g, with the ratio of amino acids superior to FAO/WHO recommend standards. The content of myoglobin was 2.22 mg/g, which was mostly in the form of oxymyoglobin. The contents of myofibrillar protein, total matrix protein, alkali-soluble protein, alkali-insoluble protein, sarcoplasmic protein and acid-soluble protein accounted for 48.35%, 17.25%, 11.84%, 10.84%, 10.04% and 1.54% of the total protein, respectively. In addition, the molecular weight of the extracted protein was 29.0-200 kDa, with 59.39% α-helix, 34.92% β-sheet and 5.69% β-turn in secondary structure. Thermal denaturation temperature of myosin and actin were 49.5℃ and 83.6℃, respectively. The emulsification property of the protein was better than that of soy protein isolate, while the foaming property was reversed. The study provides a theoretical basis for further research of protein resources of Chinese soft-shelled turtle.
MA Mengjiao
,
JING Huijuan
,
FU Anwei
,
WANG Hongxin
,
LYU Wenping
. Physicochemical properties of Chinese soft-shelled turtle protein[J]. Food and Fermentation Industries, 2019
, 45(22)
: 110
-116
.
DOI: 10.13995/j.cnki.11-1802/ts.021309
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