Using Paenibacillus campinasensis SK13. 001 genome as a template, a gene encoding β-cyclodextrin glycosyltransferase (β-CGTase) was obtained by PCR amplification. The gene fragment was cloned into the expression vector pET-22b (+), and transformed into Escherichia coli BL21 (DE3). After fermentation, the cyclization activity of the enzyme was 15 U/mL. The reaction conditions for the conversion of starch to cyclodextrin by this enzyme were optimized by single factor experiments. The results showed that using 30 g/L corn starch (mass fraction) as the substrate, pH 7. 0 phosphate buffer as the reaction solution, adding 5 U/g dry starch of this enzyme, reacted at 55 ℃ for 8 h, the conversion rate of cyclodextrin reached 45. 63%. The mass ratio of three cyclodextrins was α∶β∶γ=7∶75∶18. By optimizing the process conditions of the enzyme reaction, it provides an approach for the industrial production of cyclodextrin.
YAO Xiaolin
,
ZHANG Tao
,
JIANG Bo
. Expression of β-cyclodextrin glucosyltransferase from Paenibacillus campinasensis SK13.001 in Escherichia coli and the optimization of reaction conditions[J]. Food and Fermentation Industries, 2020
, 46(12)
: 153
-157
.
DOI: 10.13995/j.cnki.11-1802/ts.023621
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