This paper aims to provide reference for college undergraduate professional courses design. The teaching design of the section “Molecular typing of prokaryotes and its practical application” in the elective course of biology undergraduate major in colleges and universities. This paper focused on improving teacher-students interaction and teacher′s guidance ability. It can effectively motivate students to take the active study ability and the & quot;ability of apply learning & quot;. And through the diversified teaching methods and a number of vivid practical examples of assisted teaching, to help students better grasp and understand the key and difficult knowledge of this section. It can effectively improve the ability of students to analyze and solve problems using the knowledge acquired in the course. This paper has important reference value for the design and development of college biology undergraduate related courses.