In order to screen probiotic lactic acid bacteria strains with antioxidant and antimicrobial abilities, 18 strains of lactic acid bacteria preserved in our laboratory were analyzed for their acid and bile tolerance, hydrophobicity, free radical scavenging ability and antimicrobial abilities. The 16S rDNA and homology analysis of pheS and rpoA sequences were used to identify the selected strains. The results showed that strains A1, A7, A13, A14 and A15 possessed good acid and bile resistance abilities. Their survival rates were over 70% and 40% under pH 3.0 and the mass fraction of bile salt of 0.3% respectively. Moreover, strain A15 had the highest acid production capacity at pH 3.68 for 24 h. Strains A1, A7, A14 and A15 were all highly hydrophobic. The hydroxyl radicals and DPPH scavenging ability of intact cell suspension of strain A15 were 39% and 51% respectively, which were the highest in the 18 strains and followed by strain A14. In addition, only strain A15 can inhibited the growth of Staphylococcus aureus and Escherichia coli. The strain characterization by sequences homology analysis of 16S rDNA and housekeeping genes pheS and rpoA showed that A1, A7, A14 and A15 were Enterococcus lactis, Lactobacillus paracasei, Lactobacillus paraplantarum and Lactobacillus plantarum subsp. Plantarum respectively. The results of this study laid a foundation for the development of novel nutritional and healthy fermented food.
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