To study the effects of acetic acid bacteria on alcohol fermentation and ester-alcohol metabolism of yeast, Acetobacter pasteurium, a common strain found in liquor production, was mixed with high ester-producing Saccharomyces cerevisiae. While keeping the inoculation amount of high ester-producing Saccharomyces cerevisiae unchanged, different amount of acetic acid bacteria suspension and acetic acid bacteria fermentation liquid were added to the sorghum juice medium. The results showed that the acetic acid bacteria suspension had little effect on alcohol fermentation and ester-alcohol metabolism. However, with increasing in the acetic acid concentration (0~6 g/L), the amount of acetate and higher alcohols decreased gradually, and alcohol fermentation remained unchanged. Specifically, the acetate and higher alcohols decreased by up to 60% and 50%, respectively. When the acetic acid concentration reached 8 g/L, the alcohol fermentation and ester-alcohol metabolism were severely inhibited, and ethanol production dropped by 85%. In addition, at the initial acetic acid concentration of 6 g/L, 394 genes were up-regulated and 282 genes were down-regulated in the high ester-producing Saccharomyces cerevisiae. These genes were mainly encoded for cell membrane components and physiological function, antibiotic synthesis, secondary metabolites synthesis, carbon metabolism, TCA cycle, amino acids synthesis and other metabolic pathways.
ZHANG Huadong
,
GUO Xuewu
,
XIAO Dongguang
. Effect of acetic acid bacteria on alcohol fermentation and metabolism of high ester-producing Saccharomyces cerevisiae[J]. Food and Fermentation Industries, 2020
, 46(1)
: 36
-42
.
DOI: 10.13995/j.cnki.11-1802/ts.022251
[1] 王斌,陈福生.醋酸菌的分类进展[J].中国酿造,2014,33(12):1-10.
[2] 侯小歌,杜红阳,李学思,等.宋河大曲中醋酸菌的分离鉴定及产酸特性[J].中国酿造,2011,30(4):112-115.
[3] 李玮黎.西凤酒酒醅中乙酸菌的分离鉴定及其生理生化特性研究[D].杨凌:西北农林科技大学,2014.
[4] 王文晶,朱会霞,李泽霞,等.白酒固态发酵酒醅中微生物研究概况[J].酿酒,2016,43(2):27-32.
[5] 郭敏.基于高通量测序对酱香大曲制曲微生态多样性的研究[D].贵阳:贵州大学,2018.
[6] 郑宇,姜春悦,陈兴京,等.醋酸对巴氏醋杆菌生长和代谢活性的影响[J].现代食品科技,2014,30(9):149-153.
[7] 肖冬光.白酒生产技术[M].第二版.北京:化学工业出版社, 2011:31-38.
[8] 吴轩德,李洲,周世水.白酒酿造中酿酒酵母与巴氏醋杆菌相互作用的研究[J].现代食品科技,2017,33(12):61-67.
[9] 覃莉,王志,陈雄,等.酵母菌醋酸菌混菌发酵高产醋酸工艺研究[J].中国酿造,2012,31(1):144-147.
[10] 王雪山,杜海,徐岩.清香型白酒发酵过程中微生物种群空间分布[J].食品与发酵工业,2018,44(9):1-8.
[11] 王世伟,王卿惠,芦利军,等.白酒酿造微生物多样性、酶系与风味物质形成的研究进展[J].农业生物技术学报,2017,25(12):2 038-2 051.
[12] 王鹏昊,关统伟,张习超,等.小曲中优质产酯酵母分离鉴定及其产酯条件优化[J].食品与发酵工业,2018,44(2):62-67.
[13] 李岭卓.白云边酒酿造过程中优势微生物相互作用的研究[D].武汉:湖北大学,2018.
[14] 游玲,任羽,王涛,等.酵母对浓香型白酒糟醅中高级醇生成的影响[J].食品与发酵工业,2016,42(2):23-28.
[15] 邱并生.混菌发酵对白酒液态发酵效率和风味物质的影响[J].微生物学通报, 2014, 41(7):1 477-1 478.
[16] 张艳英,肖冬光,张翠英,等.BAT2缺失酿酒酵母基因工程安全菌株的构建及其杂交育种[J].食品与发酵工业,2012,38(1):36-40.
[17] 杨林娥,彭晓光,杨庆文,等.斐林试剂法测定还原糖方法的改进[J].中国酿造, 2010, 29(5):160-161.
[18] 朱明光,史媛英.血球计数板法测定酵母数和出芽率时应注意的问题[J].啤酒科技, 2001, (9):38-38.
[19] 周德庆.微生物学实验教程[M].第三版.北京:高等教育出版社, 2006:91-95.
[20] MATSUSHITA K,INOUE T,ADACHI O,et al. Acetobacter aceti possesses a proton motive force-dependent efflux system for acetic acid[J]. Journal of Bacteriology, 2005, 187(13):4 346-4 352.
[21] LASKO D R,ZAMBONI N,SAUER U. Bacterial response to acetate challenge: a comparison of tolerance among species.[J]. Applied Microbiology and Biotechnology,2000,54(2):243-247.