Food and Fermentation Industries >

2020 , Vol. 46 >Issue 1: 43 - 49

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.022753

Real-time quantitative PCR analysis of peptidoglycan hydrolases relative expression of high autolysis Lactobacillus casei mutant induced by nitrogen ion implantation

  • CUI Wenming ,
  • LYU Jiaping ,
  • WANG Xiaopeng ,
  • ZHAO Lijun ,
  • ZHU Chaozhi ,
  • ZHANG Qiuhui ,
  • LI Miaoyun ,
  • ZHAO Gaiming
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  • 1(College of Food Science and Technology, Henan Agricultural University, Zhengzhou 450002, China)
    2 (Key Laboratory of Agro-product Processing and Quality Control, Institute of Agro-food Science and Technology, Chinses Academy of Agricultural Science, Beijing 100193, China)

Received date: 2019-07-22

  Online published: 2020-03-27

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Abstract

Lactobacillus casei 116-a was induced by low energy nitrogen ion implantation to screen the high autolysis mutant and investigate differential expression of N-acetylmuramidase and N-acetylglucosaminidase between wild type strain and mutant. The results indicated that a high autolysis mutant with good genetic stability was obtained by N+ implantation with a dose of 0.5×1015 ions/cm2 under 30 keV ion energy. By comparison with the autolysis rate of the wild type strain, the autolysis rate of mutant was increased by 25.7% and up to 56.3%. Quantitative real-time PCR analysis of the expression of N-acetylglucosaminidase and N-acetylmuramidase between mutant and wild type strain revealed the expression of N-acetylglucosaminidase of mutant was up-regulated by about 55 times while the expression of N-acetylmuramidase of mutant was up-regulated by only 2.8 times. Therefore, N-acetylglucosaminidase plays an important role in Lactobacillus casei 116-a autolysis and its gene expression upregulation can effectively improve cell autolysis.

Key words： Lactobacillus casei; autolysis; ion implantation; peptidoglycan hydrolase

Cite this article

CUI Wenming , LYU Jiaping , WANG Xiaopeng , ZHAO Lijun , ZHU Chaozhi , ZHANG Qiuhui , LI Miaoyun , ZHAO Gaiming . Real-time quantitative PCR analysis of peptidoglycan hydrolases relative expression of high autolysis Lactobacillus casei mutant induced by nitrogen ion implantation[J]. Food and Fermentation Industries, 2020 , 46(1) : 43 -49 . DOI: 10.13995/j.cnki.11-1802/ts.022753

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