Isolation of endophytic bacteria from Cinnamomum longepaniculatum and screening and mutagenesis of strain with high-yield α-terpineol

  • HUANG Jinfeng ,
  • WANG Xin ,
  • LIANG Yujuan ,
  • YANG Liyan ,
  • LUO Sican ,
  • ZOU Yue ,
  • WEI Qin ,
  • LIANG Hanqiao
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  • 1(School of Food and Biotechnology, Xihua University, Chengdu 610039, China)
    2(School of Life Science and Food Engineering, Yibin University, Yibin 644000, China)
    3(Biochemical School, Beijing City University, Beijing 100094, China)

Received date: 2019-08-18

  Online published: 2020-03-27

Abstract

Screening for microorganisms from aromatic plants is an important way to produce natural fragrances using biotechnology. Endophytic bacteria were isolated from five different strains of Cinnamomum longepaniculatum (Gamble) N. Chao leaves with various oil production rates and 1,8-cineole content. Using tissue block, grinding, and neutralizer auxiliary methods with lysogeny broth culture medium (LB), tryptose soya agar medium (TSA), and lecithin Tween 80 nutrient agar medium, endophytic bacteria were isolated and grown. Gas Chromatography-Mass Spectrometer (GC-MS) were used as quantitative analysis tool to screen for high α-Terpineol -yield strains and the high-yield strains were further mutated by UV. After removing the duplicate strains according to the morphological characteristics of the colony, 176 strains of endophytic bacteria in the leaves of Cinnamomum longepaniculatum (Gamble) N. Chao were obtained. These strains were divided into 4 phyla, 13 genera, and 34 species. The Bacillus was the first dominant class. Among the 34 strains of endophytic bacteria, the optimal mutagenicity of the strain T3-M-39 with high α-Terpineol production was obtained using a 30 W UV lamp with 30 cm irradiation for 2 minutes, which had a mortality rate of 74.1%. The production of α-Terpineol was enhanced with the mutant strain, reaching 1.45 mg/L, which was increased by 34.9% compared with that of the wild strain. In addition, this mutant strain was also able to maintain its stable genetic characteristics. This finding provided the basis for the natural α-Terpineol production using biotechnology.

Cite this article

HUANG Jinfeng , WANG Xin , LIANG Yujuan , YANG Liyan , LUO Sican , ZOU Yue , WEI Qin , LIANG Hanqiao . Isolation of endophytic bacteria from Cinnamomum longepaniculatum and screening and mutagenesis of strain with high-yield α-terpineol[J]. Food and Fermentation Industries, 2020 , 46(1) : 75 -81 . DOI: 10.13995/j.cnki.11-1802/ts.022018

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