This study is aiming to screen factors that can improve the suitability of irradiated tryptic soy agar (TSA) media. Staphylococcus aureus (S. aureus) ATCC 6538 and Bacillus ginsengihumi (B. ginsengihumi) were used to study the effects of storage time and different auxiliary additives on the applicability of irradiated TSA medium. In addition, the response surface methodology (RSM) was used to optimize the crude enzyme fermentation broth of Bacillus firmus (B. firmus) GL3. At the same time, the effects of the optimal crude enzyme fermentation broth of B. firmus GL3 on the irradiated medium were explored. The results showed that S. aureus and B. ginsengihumi resumed normal growth when the media were stored for 15 d or certain additives were added. By comparing the various auxiliary additives, pyrogallic acid, gallic acid monohydrate, L-cysteine, tyrosine, phenylalanine, metal ions Mg2+, Mn2+, K+ and crude enzyme fermentation broth of catalase-producing strains can promote the growth of sensitive strains. Under optimal conditions, the activity of catalase produced by B. firmus GL3 was increased by 78.84%. Further, the optimal crude enzyme solution of B. firmus GL3 had the best growth-promoting effect on sensitive bacteria. This study provided reference for improving the applicability of irradiated TSA medium.
XU Ling
,
NING Xibin
. Improved applicability of irradiated medium and the effect of catalase produced by Bacillus firmus GL3 on its applicability[J]. Food and Fermentation Industries, 2020
, 46(4)
: 99
-105
.
DOI: 10.13995/j.cnki.11-1802/ts.022815
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