In this study, UPLC-ESI-QQQ-MS and UPLC-ESI-MS/MS techniques were used to determine the anthocyanins and non-anthocyanins in the peel of Pinot Noir grape in two representative wine grape planting bases (38 °, Mogao) in Wuwei area of Gansu province. The results showed that from the beginning of whole color conversion to the end of harvest stage, the content of total anthocyanin was showing a fluctuation change (decreased at first, then increased and decreased finally), and the content was relatively high after four weeks of color conversion. The content of total non-anthocyanins was contrary to that of total anthocyanins, and reached the maximum two weeks after color conversion. The results of correlation analysis showed that there was a high correlation between sugar-acid ratio and phenols during ripening, and a high negative correlation between total anthocyanins and total non-anthocyanins. The change of phenolic acid also influenced the accumulation of flavanols and flavonols. The results of this experiment could provide data support for local accurate judgment of harvest time, classification and guidance of planting regulation, as well as the distribution of anthocyanins and non-anthocyanins contents in grape. Meanwhile, the law of metabolic synthesis and the interaction mechanism between different types of phenolic compounds was also indicated in this research.
SHI Xiao
,
ZHANG Bo
,
NIU Jianming
,
LIU Qi
,
LI Ningning
,
CHEN Xinran
,
WANG Kaili
,
MA Tengzhen
,
HAN Shunyu
. Determination of polyphenols in ‘Pinot Noir’ grape at different ripening stages in Wuwei area of Gansu province[J]. Food and Fermentation Industries, 2020
, 46(4)
: 258
-265
.
DOI: 10.13995/j.cnki.11-1802/ts.022497
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