In order to explore whether the mould isolated from the traditional Xuanwei ham can be used as a starter culture, the mould Cladosporium cladosporioides P27 was inoculated to dry-cured sausage to investigate the effects of Cladosporium cladosporioides on physicochemical properties, oxidation level and flavor of the sausage. There was no significant difference in the pH and water activity between the inoculated group and the control group, but the total number of bacteria was significantly reduced. Besides, Cladosporium cladosporioides inoculation improved the texture of sausages by reducing hardness and increasing elasticity. Additionally, the degree of lipid and protein oxidation of the inoculated group was lower than that of the control group, which indicated that Cladosporium cladosporioides had certain antioxidant capacity. Finally, a total of 23 volatile compounds of dry-cured sausage after 30 days of fermentation were detected by GC-MS. The inoculation group had higher contents of alcohols, esters, ketones and etc than those of the control. Overall, the results of this study showed that Cladosporium cladosporioides could reduce the number of bacteria and oxidation levels, improve the texture,and enrich the flavor of sausages. Therefore, it can be used as a potential starter culture in the fermented meat products manufacture in the future.
CAI Jiaming
,
WANG Jihui
,
TAO Ye
,
XIAO Shan
,
LIU Bingnan
,
WANG Liang
. The effects of mould starter on the physicochemical parameters, oxidationdegree and flavor of dry-cured sausage[J]. Food and Fermentation Industries, 2020
, 46(5)
: 17
-22
.
DOI: 10.13995/j.cnki.11-1802/ts.022880
[1] 卢士玲,吴桂春,李开雄.发酵肉制品中乳酸菌的分离鉴定技术的研究[J].肉类工业,2005(11):16-21.
[2] TOMÁS B,SANZ Y,M-CONCEPCIÓN A,et al.Protease B from Debaryomyces hansenii: purification and biochemical properties[J]. International Journal of Food Microbiology, 2005, 98(2):167-177.
[3] 巩洋,孙霞,杨勇,等.混合菌种发酵生产的低酸度川味香肠挥发性成分分析[J].食品与发酵工业,2015,41(7):175-181.
[4] 陈杰.金华火腿成熟过程中霉菌菌相构成、安全性及与其品质关系的研究[D]. 杭州:浙江工商大学, 2007.
[5] 刘晓丽.发酵肉制品中发酵剂的研究[J].四川食品与发酵, 2002, 38(4):27-30.
[6] LEISTNER L, AYRES J C. Molds and meats[J]. Fleischwirtschaft, 1968(1):62-65.
[7] GB 5009.237—2016,食品pH值的测定[S].北京:中国标准出版社,2016.
[8] GB 4789.2—2016,食品微生物检验菌落总数测定[S].北京:中国标准出版社,2016.
[9] MARCOS B,AYM ERICH T,GUARDIA M D.et a1.Assessment of high hydrostatic pressure starter culture on the quality properties of low-acid fermented sansages[J].Meat Science,2007,76(1):46-53.
[10] GB 5009.5—2016,食品中蛋白质的测定[S].北京:中国标准出版社,2016.
[11] LUND M N,HVIID M S,CLAUDI-M AGNUSSEN C.et a1.Efects of dietary soybean oil on lipid and protein oxidation in pork patties during chill storage[J].Meat Science,2008,79(4):727-733.
[12] 李鑫,刘登勇,李亮,等.SPME-GC-MS法分析金华火腿风味物质的条件优化[J].食品科学,2014,35(4):122-126.
[13] 陈乃阳.洋葱皮提取物对西式香肠抗氧化及食用品质影响研究[D].延边:延边大学,2017.
[14] 侯新月,卢士玲,郭新,等.羊肉火腿在加工过程中脂肪变化的研究[J].食品工业,2017,38(10):34-37.
[15] RAMI H,THIERRY A,ANDRÉ L,et al. Quantitative study of the relationships among proteolysis, lipid oxidation, structure and texture throughout the dry-cured ham process[J]. Food Chemistry,2015,166:522-530.
[16] 王建辉,刘永乐,刘冬敏,等.冷藏期间草鱼鱼片脂肪氧化变化规律研究[J].食品科学,2013,34(6):251-254.
[17] GE Q,CHEN S,LIU R,et al.Effects of Lactobacillus plantarum NJAU-01 on the protein oxidation of fermented sausage[J]. Food Chemistry, 2019, 295:361-367.
[18] FUENTES,VERÓNICA,ESTÉVEZ,et al.Impact of lipid content and composition on lipid oxidation and protein carbonylation in experimental fermented sausages[J]. Food Chemistry,2014,147:70-77.
[19] 李晋,杨显辉,夏河山,等.金华火腿挥发性风味物质研究进展[J].青海畜牧兽医杂志,2016,46(2):55-57.
[20] 李想,汪志君,于海.干腌火腿的加工工艺及其品质的影响因素[J].食品科技,2010(2):114-117.
[21] CARROLL A L,DESAI S H,ATSUMI S.Microbial production of scent and flavor compounds[J].Current Opinion in Biotechnology, 2016, 37:8-15.
[22] CORRAL S,SALVADOR A,FLORES M.Salt reduction in slow fermented sausages affects the generation of aroma active compounds[J].Meat Science,2013,93(3):776-785.
[23] BERGER R G.Biotechnology as a source of natural volatile flavours[J]. Current Opinion in Food Science,2015,1(4):38-43.
[24] CHEN SHUANG,SHA SHA,QIAN MICHAEL,et al.Characterization of volatile sulfur compounds in Moutai Liquors by headspace solid-phase microextraction gas chromatography-pulsed flame photometric detection and odor activity value[J].Journal of Food Science,2017,82(12):2816 -2822.
[25] 敖锐,彭茵,陈仁远,等.酱香型白酒的勾调和调味酒的运用[J].酿酒科技,2016(12):74-76.
[26] GB 2760—2014,食品添加剂使用标准[S]. 北京:中国标准出版社,2014.
[27] 牛爽,乔发东,徐静,等.发酵干香肠中挥发性成分的分析[J].食品与发酵工业,2005,31(2):101-104
[28] 汪淼,于海,吴满刚,等.发酵肉制品风味形成研究进展[J].食品工业,2014,35(9):221-226
[29] ASUMING-BEDIAKO N, JASPAL M H, HALLETT K, et al. Effects of replacing pork backfat with emulsified vegetable oil on fatty acid composition and quality of UK-style sausages[J]. Meat Science, 2014, 96(1):187-194
[30] 蒋莹,刘欣.柠檬烯的开发利用研究进展[J].食品安全导刊,2018(9):133.
[31] 罗琴,聂波,宋志远,等.产3-羟基丁酮菌株的分离、筛选与鉴定[J].安徽农业科学,2019,47(9):1-3;7.