A uric acid oxidase-producing strain was screened from the human intestinal tract and identified as Proteus penneri JC-5 by morphological observation and molecular biology (16S rDNA sequencing). The enzymatic properties of the uric acid enzyme produced by JC-5 were studied. According to the experimental results, the optimum temperature of the enzyme was 35 ℃, and the thermal stability was poor; the optimum pH was 8.0, and it had better stability under weak alkaline conditions; Ca2+ and Mn2+ had activation effect on the enzyme, and Ca2+ showed better activation than Mn2+; Fe2+, K+, Mg2+, Cu2+, Zn2+ showed inhibitory effects on the enzyme activity.
ZHANG Qingfang
,
WANG Junchen
,
YU Shuang
,
LIU Chunying
,
CHI Xuemei
,
CHI Naiyu
. Screening, identification and enzymatic properties of uric acid oxidase-producing bacteria in human intestines[J]. Food and Fermentation Industries, 2020
, 46(8)
: 34
-39
.
DOI: 10.13995/j.cnki.11-1802/ts.023299
[1] 开雷,岳珂,马晓航.尿酸氧化酶的研究及其应用[J].科技通报,2008,24(2):183-187.
[2] FINI M A,STENMARK K R. Pegloticase and lowering blood pressure in refractory gout; is it uric acid or hydrogen peroxide?[J]. European Journal of Internal Medicine,2019,69:e11-e12.
[3] ZHANG Ran,GAO Shijue,ZHU Chunyan,et al. Characterization of a novel alkaline Arxula adeninivorans urate oxidase expressed in Escherichia coli and its application in reducing uric acid content of food.[J]. Food chemistry,2019,293:254-262.
[4] ZHUANG Miaomiao,ZHANG Yanwen,ZHOU Shaohong,et al. Uricase-containing coacervate microdroplets as enzyme active membrane-free protocells for detoxification of uric acid in serum[J]. Chemical communications ,2019,55(92):13 880-13 883.
[5] IMANI M, SHAHMOHAMADNEJAD S. Recombinant production of Aspergillus Flavus uricase and investigation of its thermal stability in the presence of raffinose and lactose[J]. 3 Biotech, 2017, 7(3):201.
[6] SORENSEN L B. Role of the intestinal tract in the elimination of uric acid[J]. Arthritis & Rheumatism:Official Journal of the American College of Rheumatology,1965,8(4):694-706.
[7] LEY R E, PETERSON DA, GORDON J I. Ecological and evolutionary forces shaping microbial diversity in the human intestine[J]. Cell,2006,124(4):837-848.
[8] 金钗,徐明智.肠道菌群与高尿酸血症及痛风的相关性研究[J].中国微生态学杂志,2019,31(8):980-984.
[9] MIELKE S T, HEWETT G E. Assay device and method using a signal-modulating compound:U.S.Patent5,156,954[P].1992-10-20.
[10] 宋铖铖. 猪肺血管紧张素转化酶分离纯化及酶活性研究—中国食品科学技术学会第十五届年会论文摘要集[C].山东:中国食品科学技术学会,2018:530.
[11] SCHWARZ M,LOSSOW K,KOPP J F,et al. Crosstalk of Nrf2 with the trace elements selenium, iron, zinc, and copper[J]. Nutrients,2019,11(9):2 110.
[12] 宁豫昌,张晓静,乔宏兴.猪肠道中产蛋白酶解淀粉芽孢杆菌的筛选、鉴定及其酶学性质的初步研究[J].中国畜牧兽医,2017,44(8):2 398-2 407.
[13] 咸静女,郭鑫,李波,等. 微生物尿酸氧化酶的筛选、酶学性质及重组表达[J]. 生物工程学报, 2018, 34(7):1 147-1 155.
[14] DRZEWIECKA D. Significance and roles of proteus spp. bacteria in natural environments[J]. Microbial Ecology, 2016, 72(4):741-758.
[15] SINGH T A, DEVI K R, AHMED G, et al. Microbial and endogenous origin of fibrinolytic activity in traditional fermented foods of Northeast India[J]. Food Research International, 2014, 55: 356-362.
[16] CANTÁN R, SÓNCHEZ-MORENO M P,REILLY MIM. Proteus penneri[J]. Enfermedades Infecciosas Y Microbiología clínica. 2006,24(S1):8-13.
[17] MOBLEY H L T, JONES B D, PENNER J L. Urease activity of Proteus penneri[J]. Journal of Clinical Microbiology, 1988, 25(12):2 302-2 305.
[18] 郑丹. 尿毒症患者肠道细菌对小分子尿毒素的分解作用[D]. 长沙:中南大学,2010.
[19] PESSION A, MELCHIONDA F, CASTELLINI C. Pitfalls, prevention, and treatment of hyperuricemia during tumor lysis syndrome in the era of rasburicase (recombinant urate oxidase)[J]. Biologics Targets & Therapy, 2008, 2(1):129-141.
[20] PATTE C, SAKIROGLU O, SOMMELET D. European experience in the treatment of hyperuricemia[J]. Seminars in Hematology, 2001, 38(Suppl 10):9-12.
[21] PATEL K S, LAU J E, ZEMBILLAS A S, et al. Single 4.5 mg fixed-dose of rasburicase for hyperuricemia associated with tumor lysis syndrome[J]. Journal of Oncology Pharmacy Practice, 2017,23(5):333-337.
[22] KAI L, MA X H, ZHOU X L, et al. Purification and characterization of a thermostable uricase from Microbacterium sp. strain ZZJ4-1[J]. World Journal of Microbiology & Biotechnology, 2008, 24(3):401-406.
[23] 张庆芳,逄飞,于爽,等.海洋高产尿酸氧化酶菌株筛选鉴定及酶学性质研究[J].生物技术通报,2019,35(7):61-69.
