In order to explore a new approach for selenium-rich food and preparation of selenium-enriched probiotics, five Lactobacillus strains were treated with different concentrations of sodium selenite. One Lactobacillus strain was screened by observing the bacteria condition and the colony number. The conditions of selenium enrichment were optimized by single factor experiment and response surface method, the activity of lactic acid bacteria was evaluated after selenium enrichment optimization. The Lactobacillus plantarum was screened as the selenium-rich dominant bacteria. The optimal conditions for selenium enrichment were as follows: NaCl 2 g/100mL, sodium selenite 16 μg/mL, inoculation amount 3%, and selenium enrichment rate 84.26%. Scanning electron microscopy (SEM) showed that the selenium-rich L. plantarum didn’t deform, and selenium was generated on the surface after enrichment. After digestion in simulated gastrointestinal, the number of viable bacteria of se-enriched L. plantarum was above 7 lgCFU/mL, and the survival rate was significantly higher than that of the non-selenium-enriched group (P <0.05), and could play a better functional and probiotic role, providing experimental data support for the subsequent study of selenium-rich lactic acid bacteria fermentation food.
YI Xin
,
ZHOU Qi
,
OUYANG Zhu
,
TAN Anqun
,
FAN Jiaying
,
LI Zeling
,
ZHU Xiajian
,
HUANG Linhua
,
LI Guijie
,
WANG Hua
. Selenium enrichment optimization of lactic acid bacteria and evaluation of its activity[J]. Food and Fermentation Industries, 2020
, 46(8)
: 179
-186
.
DOI: 10.13995/j.cnki.11-1802/ts.023276
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