The aim of this study was to establish an ultra-fast liquid chromatography-mass spectrometry (UFLC-MS/MS) method for the analysis and evaluation of 31 amino acids and nucleosides in peppermint in order to provide scientific knowledge for the comprehensive utilization of mint resources. The UFLC-MS/MS parameters were as follows: Waters XBridge Amide column (100 mm×2.1 mm, 3.5 μm); mobile phase 0.2% formic acid -0.2% formic acid acetonitrile, gradient elution, flow rate 0.2 mL/min, column temperature 30 ℃, positive Ion multiple reaction monitoring (MRM) mode determination. PCA and TOPSIS methods were used for the comprehensive evaluation of the samples. The 21 amino acids and 10 nucleosides showed a good linear relationship with the peak area within a certain concentration range (r2>0.999 0). The precision, repeatability and stability of the method were good, and the RSD values were all higher than 3.85%. The recovery rate of the sample was between 94.18% and 98.76% and the RSD was between 1.17% and 3.56% respectively. Peppermint is rich in amino acids and nucleosides and the content varies significantly from different regions. The quality of peppermint samples from Fuyang was the best. In summary, this method developed here is accurate and efficient and the results could provide a reliable basis for the quality evaluation and comprehensive utilization of the dual-use mint.
SUN Huijuan
,
WANG Rui
,
SONG Qianqian
,
ZHOU Huiyin
,
FANG Chengwu
. The analysis of amino acid and nucleoside components in medicinal and edible Mentha haplocalyx based on UFLC-MS/MS technology[J]. Food and Fermentation Industries, 2020
, 46(8)
: 261
-266
.
DOI: 10.13995/j.cnki.11-1802/ts.023495
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