The mutagenesis of Bacillus subtilis B-0 was carried out using atmospheric and room temperature plasma (ARTP) and 5-bromouracil (5-BU), and the adenosine high-yield strain was selected by sulfaguanidine (SG) resistance and in 48-well plates. A genetic stable mutant strain B-6 with 35% higher yield of adenosine than that of Bacillus subtilis B-0 was finally obtained after 6 cycles of mutagenesis and screening. Briefly, the combination of ARTP and 5-BU mutagenesis combined with 48-well plates can significantly improve the breeding efficiency and accuracy of adenosine high-yielding strains, and also provide a theoretical reference for the selection of other nucleoside strains.
YANG Xinping
,
SONG Ci
,
ZHANG Weihao
,
LIU Yan
,
WANG Zhou
,
XUE Zhenglian
. Combined mutagenesis of ARTP and 5-BU for improving production ofadenosine in Bacillus subtilis[J]. Food and Fermentation Industries, 2020
, 46(9)
: 73
-77
.
DOI: 10.13995/j.cnki.11-1802/ts.023169
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