Food and Fermentation Industries >

2020 , Vol. 46 >Issue 12: 73 - 79

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.023519

Isolation, purification, structural characterization and antioxidant activity of polysaccharide from Zingiber officinale Roscoe

  • SONG Lili ,
  • WEN Ge ,
  • HUO Shanhao ,
  • HU Xiaolong ,
  • PENG Hui
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  • 1(School of Food and Bio-engineering, Zhengzhou University of Light Industry, Zhengzhou 450001, China)
    2(HeNan Provincial People′s Hospital, Zhengzhou 450003, China)
    3(Postdoctoral Research & Development Base, Yangshao Distillery Co. Ltd., Sanmenxia 472000, China)

Received date: 2020-02-05

  Online published: 2020-07-15

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Abstract

The polysaccharide was extracted from Zingiber officinale Roscoe through complex enzymatic hydrolysis combined with hot-water extraction and subjected to Sevage deprotein, dialysis, ethanol precipitation, DEAE-52 cellulose ion-exchange column chromatographic analysis and Sephadex G-100 column chromatographic analysis, and the antioxidant activity in vitro was further studied. Results showed that the polysaccharide of Zingiber officinale did not contain impurities such as nucleic acid and protein. The polysaccharide had a typical characteristic absorption peak of polysaccharide, which was β - pyran type polysaccharide with a triple helix structure. The polysaccharide had thermal stability and its molecular weight was (3.72±0.23)×105 Da. Results of antioxidant activity in vitro showed that the highest scavenging capacity against hydroxyl radicals, superoxide radicals, ABTS and DPPH was (65±2)%, (37±3)%, (96±4)% and (83±3)%, respectively, which had a good antioxidation activity in vitro. This study provides a theoretical basis for the exploitation and utilization of Zingiber officinale, and provides the research basis for the development of Zingiber officinale polysaccharide in the field of functional food and medicine.

Key words： Zingiber officinal Roscoe; polysaccaride; structural characterization; antioxidation activity

Cite this article

SONG Lili , WEN Ge , HUO Shanhao , HU Xiaolong , PENG Hui . Isolation, purification, structural characterization and antioxidant activity of polysaccharide from Zingiber officinale Roscoe[J]. Food and Fermentation Industries, 2020 , 46(12) : 73 -79 . DOI: 10.13995/j.cnki.11-1802/ts.023519

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