A simple, efficient and sensitive method for the determination of α-solanine and α-chaconine in potato was established. Samples were extracted with acidified methanol and then absorbed with excess water by anhydrous sodium sulfate and magnesium acetate. After centrifugation, the acetonitrile and formic acid ammonium buffer solution were mobile phase, gradient elution, the flow rate was 0.4 mL/min, the column temperature was 40 degrees, the injection volume was 5μL, the extract was separated by chromatographic column, and the multi reaction analysis was carried out by four level mass spectrometer under the electrospray ionization mode. In the experimental concentration range, the standard curves of α-solanine and α-chaconine are well linear, and the correlation coefficient (R2) is greater than 0.999; at the concentration levels of 10, 50 and 100 ng/mL, the recoveries of the two analytes are 98.9%-101.6%, the relative standard deviation is 0.18%-1.41%, the detection limit and quantitative limit are 0.3 mg/kg and 1.0 mg/kg respectively. The sample was analyzed and compared with the pre-treatment extraction results reported in the literature. This method can achieve better results than the known methods. Compared with the supplementary method, the recovery rate of this method is much higher than that of the supplementary method, which further proves the feasibility of this method. The results showed that the method was simple, reproducible and accurate, and could be used for the analysis and monitoring of the major glycoside alkaloids in potato.
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