As an important nutritional essential amino acid,L-tyrosine was widely used in food,medical,cosmetics and other industries. The non-phosphotransferase glucose transport pathway was a key way for bacteria to absorb glucose,which had significant effect on the metabolism of bacteria. In this study,the Bacillus amyloliquefaciens HZΔptsH was used as the original strain. Different genes of glucose transporters were expressed to strengthen the glucose absorption transport,and their effects on the biosynthesis of L-tyrosine were investigated. Flasks fermentation results showed that the yield of L-tyrosine was improved by 22% after expression of the native glucose transporter gene glcP in HZΔptsH. Wherein,overexpressing the galP gene from Escherichia coli and idolT1 gene from Corynebacterium glutamicum showed no significant effect on L-tyrosine production. This study confirmed that overexpressing the native glcP gene of B. amyloliquefaciens was an effective strategy for enhancing L-tyrosine production,which provided the reference for further metabolic engineering breeding of B. amyloliquefaciens for L-tyrosine synthesis.
JI Anying
,
WEI Xuetuan
. Engineering of the non-phosphotransferase glucose transport system for enhancing L-tyrosine production in Bacillus amyloliquefaciens[J]. Food and Fermentation Industries, 2020
, 46(15)
: 27
-31
.
DOI: 10.13995/j.cnki.11-1802/ts.023863
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