Food and Fermentation Industries >

2020 , Vol. 46 >Issue 15: 52 - 59

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.023864

Analysis of ACE-inhibitory peptides in hard cheese made from yak milk and its hydrolysate after in vitro gastrointestinal digestion

  • SONG Xuemei ,
  • LIANG Qi ,
  • SONG Guoshun ,
  • ZHANG Yan
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  • 1(College of Food Science and Engineering,Gansu Agricultural University,Lanzhou 730070,China)
    2(Engineering Laboratory of Functional Dairy of Gansu Province,Lanzhou 730070,China)

Received date: 2020-03-05

  Revised date: 2020-04-15

  Online published: 2020-08-15

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Abstract

The water-soluble extract (WSE) of hard cheese made from yak milk was subjected to in vitro simulated gastrointestinal digestion using pepsin and trypsin. The water-soluble extract and its generated hydrolysates were tested for angiotensin-converting enzyme (ACE) inhibitory activity and ACE-inhibitory peptides of WSE and the hydrolysate were identified using capillary liquid chromatography coupled to Quadrupole-Orbitrap mass spectrometry. The results showed that fifteen and twelve peptides were respectively identified in WSE and its hydrolysate. Majority of peptides belonged to β-casein (β-CN) and their molecular weight ranged from 800 Da to 1 500 Da. Eighteen peptides of the above sequences had feature in common with the C-terminal tripeptide sequence of other ACE-inhibitory peptides reported in the literature,many ACE-inhibitory peptides had a structure-characteristic which contributes to inhibit ACE and they were potential ACE-inhibitory peptides. Six peptides with previously demonstrated ACE-inhibitory,which sequences were SKVLPVPQ,QEPVLGPVRGPFP and YQEPVLGPVRGPFPI in WSE,and LVYPFPGPIPN,VYPFPGPIPN and YQEPVLGPVR in WSE digestant,were found. ACE inhibitory activity of WSE of cheese was not significant different from that of in vitro gastrointestinal digestants of its WSE (P>0.05). The research provides the theoretical basis for exploiting hard cheese made from yak milk with functional characteristic.

Key words： hard cheese made from yak milk; ACE-inhibitory peptides; digestion

Cite this article

SONG Xuemei , LIANG Qi , SONG Guoshun , ZHANG Yan . Analysis of ACE-inhibitory peptides in hard cheese made from yak milk and its hydrolysate after in vitro gastrointestinal digestion[J]. Food and Fermentation Industries, 2020 , 46(15) : 52 -59 . DOI: 10.13995/j.cnki.11-1802/ts.023864

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