The present study aimed to analyze the effects of exogenous fructose treatment on alleviating lignification deterioration of bamboo shoots (Phyllostachys praecox) during cold storage and the involved mechanism on basis of the regulation of sucrose metabolism. The bamboo shoots were sprayed by the fructose solutions at different concentrations (0,10,20,30 or 40 mmol/L) at 20 ℃ and air-dried respectively. Then the bamboo shoots were stored at 4 ℃ for 20 days. During the storage,the bamboo shoots were collected at a 5-day interval for measurement of the lignification,quality parameters,contents of soluble sugars,uridine diphosphate glucose (UDPG) as well as the expressions of a set of relevant genes involved in sucrose and phenylpropanoid metabolism pathway. The results exhibited that the treatment of bamboo shoots with 20 mmol/L fructose could delay the increase of firmness, the loss of weight,browning degree and the contents of lignin and cellulose. And,it could enhance the extractable juice rate and maintain the overall quality and antioxidant activity,thus contributing to the alleviation of lignification deterioration during the cold storage. Meanwhile,the fructose treatment significantly up-regulated the expression levels of PpSS2,PpSPS and PpSPP which could promote the sucrose synthesis and decrease the accumulation of UDPG in bamboo shoots during the storage. On the contrary,the expression levels of PpPAL,Pp4CL,PpCAD,PpF5H,PpCCOMT and PpPOD related to phenylpropanoid metabolism in fructose-treated bamboo shoots were down-regulated remarkably,accompanied with the clear decrease in lignin content. Therefore,it is deduced that the fructose treatment could enhance sucrose synthesis and decrease UDPG accumulation in terms of the substances for lignin synthesis,resulting in competitive inhibition of phenylpropanoid pathway for lignin synthesis and finally inhibiting the lignification deterioration in bamboo shoots during cold storage.
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