In order to explore the quorum sensing (QS) of Pseudomonas and its effect on meat spoilage, exogenous acylhomoserine lactone (AHLs) -like signal molecules were added to the culture medium of Pseudomonas to measure the growth curves and biofilm change. Sterile pork chunks were treated with Pseudomonas solution supplemented with AHLs. Sensory evaluation was carried out during storage. Total volatile basic nitrogen (TVB-N) was measured. Volatile organic compounds were analyzed by gas chromatography-ion mobility spectrometry (GC-IMS). The results showed that the four signal molecules, N-hexanoyl-L-homoserine lactone(C6-HSL), N-octanoyl-L-homoserine lactone(C8-HSL),N-dodecanoyl-L-homoserine lactone (C12-HSL) and N-tetradecanoyl-L-homoserine lactone(C14-HSL) had no obvious influence on the growth of Pseudomonas(P>0.05), but they could promote the formation of biofilm(P<0.05), of which C8-HSL and C12-HSL had the most significant influence. At the later stage of pork storage, sensory score in the group with exogenous AHLs signal molecules was significantly lower than that in the control group (P<0.05), and theTVB-N value was higher (P<0.05). The volatile organic compounds produced by adding exogenous AHLs signal molecules were different from the fresh group and the control group, and the volatiles produced were also different when adding different signal molecules. The C14-HSL group had the highest similarity with the control group, while the C6-HSL group had the lowest similarity with the control group and the fresh group. This study showed that exogenous signal molecules have no effects on the growth of Pseudomonas. However, they could promote the spoilage ability of Pseudomonas, and the effects of different signal molecules were different.
ZHANG Yuhua
,
MENG Yi
,
WANG Shunyi
,
CHEN Dongjie
. Effect of exogenous AHLs signal molecules on the spoilage ability of Pseudomonas[J]. Food and Fermentation Industries, 2020
, 46(18)
: 78
-84
.
DOI: 10.13995/j.cnki.11-1802/ts.023026
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