Combined with the content of spectroscopy, food science and materials science, spectroscopy protocols and scanning electron microscope were used to analyze the composition of calcium in fish bones of "Four Major Chinese Carps" in detail. The calcium salts of the four fish bones mainly existed in the form of crystalline apatites (hydroxyapatite and carbonate apatite) and amorphous CaHPO4, as determined by infrared spectroscopy, scanning electron microscopy, and X-ray photoelectron spectroscopy. Then, based on the Ca/P ratio in the X-ray photoelectron spectroscopy and the PO3-4/CO2-3 ratio in the Raman spectrum, the content of three calcium salts was calculated. The hydroxyapatite content of fish bones in black carp (Mylopharyngodon piceus), grass carp (Ctenopharyngodon idellus), silver carp (Hypophthalmichthys molitrix), bighead carp (Hypophthalmichthys nobilis) was 51.18%, 46.72%, 28.49% and 29.31%, respectively. The carbonate apatite contents of fish bones were 9.89% (black carp), 10.61% (grass carp), 8.60% (silver carp) and 9.72% (bighead carp), respectively. The content of amorphous CaHPO4 was 38.93% (black carp), 42.67% (grass carp), 62.91% (silver carp) and 60.97% (bighead carp), respectively. Moreover, the contents of OH- substituted by other elements in hydroxyapatite and carbonate apatite were calculated based on narrow-spectrum scanning of X-ray photoelectron spectroscopy and X-ray diffraction phase retrieval. These results may provide theoretical basis for the comprehensive utilization of fish bone calcium in “Four Major Chinese Carps”.
GUO Hongzhuang
,
HU Yueming
,
WANG Hui
,
TU Zongcai
. Composition of calcium in the bones of four major Chinese carps[J]. Food and Fermentation Industries, 2020
, 46(18)
: 226
-231
.
DOI: 10.13995/j.cnki.11-1802/ts.023336
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