The present study aims to screen ochratoxin A(OTA)-producing microbes in wine grapes and root soil, and to identify the OTA production conditions. Wine grapes and root soil from four major producing areas in Xinjiang (Yanqi basin, Tuha basin, North foot of Tianshan mountain, Yili river valley) were used for microbial separation and purification, and molecular biology identification were performed. Thus, the OTA contents were determined strains were selected using fluorescence UV primary screening and enzyme-linked immunoassay (ELISA) secondary screening, and the single-factor experiment was used to explore the OTA production conditions. 12 strains of molds were isolated, of which M2 (Aspergillus niger) and M7 (Aspergillus carbonarius) were identified as OTA-producing strains, and the yield of OTA by M7 was higher. The inoculum size, incubation temperature, and mechanical injury of the wine grapes had was significant related to OTA production capacity of A. niger.
ZHAO Hao
,
QUAN Li
,
YU Jiajun
,
ZHANG Xiaomeng
,
MA Wenrui
,
WU Yun
,
XUE Jie
. Ochratoxin A-producing microbes in wine grapes in Xinjiang: Identification and formation condition[J]. Food and Fermentation Industries, 2020
, 46(19)
: 35
-41
.
DOI: 10.13995/j.cnki.11-1802/ts.023606
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