The bacteriostatic activity of Phellodendron chinense extracts against Alternatia alternata and Penicillium expansum was studied in order to provide a basis for the development of new natural fresh-keeping agent for fruits and vegetables. Measuring mycelium growth and plate count method were used to determine the antifungal activity in vitro. In addition, the disease incidence of fruit was detected by treating cherry tomato with the extract of minimum inhibitory concentration(MIC). When the concentration of P.chinense extracts was 12 g/L, the inhibition rates for mycelial growth and spore germination of A.alternata and P.expansum were both over 80%. MICs were 25 and 12.5 g/L, the minimum fungicidal (MFC) were 50 and 25 g/L and the half maximal inhibitory concentration (EC₅₀) was 6.08 and 4.32 g/L, respectively. The P.chinense extracts had significant protective and therapeutic effects on black spot and fruit rot of cherry tomato. After 12 days of storage, the cherry tomatoes in the control group all rotted, and the disease incidence in the treatment group was significantly reduced. The control efficiency was 21.11% and 38.52%, respectively. The bacteriostasis rate of n-butanol, extracted with different organic solvents, to two kinds of pathogenic fungi was above 90%. The P.chinense extracts had good bacteriostatic effect on two kinds of pathogenic fungi of cherry tomato, which could effectively reduce the incidence of fruit diseases, and n-butanol extract was the most effective in fungistasis. These results of study provide the basis for developing new, high-efficient, natural and environment-friendly fresh-keeping agent for fruits and vegetables.