A β-fructofuranosidase gene from Arthrobacter 10137 was cloned successfully and heterologously expressed in Escherichia coli BL21.The cloned fragment contained 1488bp and the protein consisted of 496 amino acids.We also studied the different culture conditions of recombinant E.coli.The results showed that the enzyme activity attain to maximum 1 412.30U/g when induced with 0.5 mmol/L IPTG for 12h at 22℃.The molecular weight was 67 ku by SDS-PAGE identified,and the purified enzyme activity was about 2 207.48 U/g.Bioreactor culture test in 2L bioreactor showed that the activity of β-fructofuranosidase was 1 208.23U/g after inducing 10h.