A high performance liquid chromatographic method was set up on a column of RSpak KC-811(300mm×8mm i.d.)for rapid measuring the concentrations of α-ketoglutaric acid,pyruvic acid,citric acid,malic acid,fumaric acid,succinic acid,lactic acid,formic acid and acetic acid.The mobile phase was water and perchloric acid solution(4 mmol/L,pH 2.4)at a flow rate of 1 mL/L.The ratio of perchloric acid to water was controlled by a gradient elution program.The assay was carried out at a column temperature of 60℃ and detection wavelength of 210 nm.The method could effectively separate and detect 8 organic acids in 1,3-propanediol fermentation broths in 15 min.All the correlation coefficients(R2)were no less than 0.999 2.The recovery and precision(RSD)were 96.8%-102.5%,0.97%-2.72%,respectively.This precise and accurate method could be used to analyze the organic acids in 1,3-propanediol fermentation broths.