Cellulase-secreting microorganisms include fungi and a few cellulose-decomposing bacteria.In this study,human rectal microbiota were screened by using filter paper as the only carbon source,and one cellulase-secreting bacteria,H1,was successfully separated.Its cellulase-secreting activity was identified using cellulose congo-red medium,morphology identification undertaken by gram staining,and classification accomplished via molecular identification.The cellulase activity of H1 was also analyzed.The results show it is positive in the cellulose congo-red medium,short and rod-shaped and gram-negative.Cellulase activity analysis indicated that its highest cellulase activity is about 0.8 U occurred at 13 hours.Molecular identification characterized the strain as Pseudomonas specie.
Lin Qingpeng
,
Luo Jianming
,
Peng Xichun
,
Pang Qiufang
. Screening and Identification of A Cellulase-secreting Bacteria,and the Analysis of Its Cellulase-activity[J]. Food and Fermentation Industries, 2010
, 36(01)
: 49
-51
.
DOI: 10.13995/j.cnki.11-1802/ts.2010.01.011