Food and Fermentation Industries

Screening and Identification of A Cellulase-secreting Bacteria,and the Analysis of Its Cellulase-activity

  • Lin Qingpeng ,
  • Luo Jianming ,
  • Peng Xichun ,
  • Pang Qiufang
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Online published: 2010-01-25

Abstract

Cellulase-secreting microorganisms include fungi and a few cellulose-decomposing bacteria.In this study,human rectal microbiota were screened by using filter paper as the only carbon source,and one cellulase-secreting bacteria,H1,was successfully separated.Its cellulase-secreting activity was identified using cellulose congo-red medium,morphology identification undertaken by gram staining,and classification accomplished via molecular identification.The cellulase activity of H1 was also analyzed.The results show it is positive in the cellulose congo-red medium,short and rod-shaped and gram-negative.Cellulase activity analysis indicated that its highest cellulase activity is about 0.8 U occurred at 13 hours.Molecular identification characterized the strain as Pseudomonas specie.

Cite this article

Lin Qingpeng , Luo Jianming , Peng Xichun , Pang Qiufang . Screening and Identification of A Cellulase-secreting Bacteria,and the Analysis of Its Cellulase-activity[J]. Food and Fermentation Industries, 2010 , 36(01) : 49 -51 . DOI: 10.13995/j.cnki.11-1802/ts.2010.01.011

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