Food and Fermentation Industries

Isolation and Identification of A Hansenula anomala Strain from Local Wine Yeast

  • Tao Xianqing ,
  • Tao Nengguo
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Online published: 2010-01-25

Abstract

In present study,a yeast strain designated as S-1 was isolated from the local wine yeast.Morphological,physiological and biochemical assays showed that it was Hansenula anomala.After cultured on the YPD medium supplied with 0%,8%,10%,12%,14% and 16% ethanol(V/V)for 144 h at 28°C,S-1 was observed with alcoholicity endurance of 12%(V/V).The fermentation assay using 500 mL sugarcane juice with sugar degree 13°Brix revealed that 70 mL fractions were obtained after primary distillation and rectification.Results by gas chromatography(GC)analysis revealed that the fraction was pure ethanol,with a yield of 0.52 g ethanol per gram sugarcane juice,which was close to the theoretical conversion.Our present results suggested that the isolated Hansenula anomala S-1 was an ideal strain for ethanol production from the sugarcane.

Cite this article

Tao Xianqing , Tao Nengguo . Isolation and Identification of A Hansenula anomala Strain from Local Wine Yeast[J]. Food and Fermentation Industries, 2010 , 36(01) : 101 -104 . DOI: 10.13995/j.cnki.11-1802/ts.2010.01.027

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