In present study,a yeast strain designated as S-1 was isolated from the local wine yeast.Morphological,physiological and biochemical assays showed that it was Hansenula anomala.After cultured on the YPD medium supplied with 0%,8%,10%,12%,14% and 16% ethanol(V/V)for 144 h at 28°C,S-1 was observed with alcoholicity endurance of 12%(V/V).The fermentation assay using 500 mL sugarcane juice with sugar degree 13°Brix revealed that 70 mL fractions were obtained after primary distillation and rectification.Results by gas chromatography(GC)analysis revealed that the fraction was pure ethanol,with a yield of 0.52 g ethanol per gram sugarcane juice,which was close to the theoretical conversion.Our present results suggested that the isolated Hansenula anomala S-1 was an ideal strain for ethanol production from the sugarcane.