In this paper,the organic nitrogen sources of fermentation medium for Blakeslea trispora were optimized.Beer yeast hydrolysate,corn steep liquor,yeast extract,peptone were added in fermentation medium which contained basic nitrogen sources.The production of lycopene,biomass of B.trispora and the use of carbohydrate in fermetation were detected after 108 hours fermetation.The results showed that beer yeast hydrolysate can improve lycopene production and corn steep liquor can promote the growth of B.trispora.The addition of yeast extract and peptone had no distinctly influence on the production of lycopene.