The ligninolytic enzymes were separated and purified from the culture broth of Pleurotus eryngii Co60-7 strain.An efficient separation process was proposed,which was mainly composed of the precipitation of ammonium sulfate and ion exchange chromatography.In the precipitation of ammonium sulphate,two-stage precipitations were applied.In the first-stage precipitation,35% saturation of ammonium sulfate was used to remove the hybridproteins and in second-stage of precipitation,75% saturation of ammonium sulfate was used to capture the ligninolytic enzymes in activity yield of 90.8.% and purification factor 3.81.The ligninlolytic enzymes were purified by DEAE-SepharoseTM Fast Flow ion-exchange chromatography under three-step elution.The total purification process attained 75.42% activity yield with a purification factor of 10.55.Ligninolytic enzymes produced in this work were used to decolorize three dyes,i.e.bromchlorphenol blue,methylene blue and Congo red,which could be classified as triphenylmethane,heterocyclic and disazo dyes.The results showed that the structures of dyes can significantly affect decolorization.