Food and Fermentation Industries >

2020 , Vol. 46 >Issue 20: 178 - 186

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.024843

Enzymolysis-ultrasound-assisted surfactant extraction and antioxidant activity of saponins from Panax notoginseng flowers

  • CHEN Honghui ,
  • TARUN BELWAL ,
  • LI Gangfeng ,
  • LUO Zisheng
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  • 1(College of Chemistry and Engineering, Wenshan University, Wenshan 663099, China);
    2(College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058,China);
    3(Fuli Institute of Food Science, Zhejiang University, Hangzhou 310058, China);
    4(Key Laboratory of Agro-Products Postharvest Handling, Ministry of Agriculture and Rural Affairs, China National-Local Joint Engineering Laboratory of Intelligent Food Technology and Equipment, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang Engineering Laboratory of Food Technology and Equipment, Zhejiang University, Hangzhou 310058,China)

Received date: 2020-06-23

  Revised date: 2020-07-21

  Online published: 2020-11-12

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Abstract

An enzymolysis-ultrasound-assisted surfactant extraction method was developed and optimized to enhance the saponins yield from Panax notoginseng flowers. The Triton X-100 surfactant was used as extraction solvent. When the enzyme concentration was 0.2%, solvent to sample ratio at 30∶1(mL∶g) and Triton X-100 solution at pH 5, the optimal extraction condition of enzymolysis-ultrasound-assisted surfactant extraction was as follows: Enzymolysis temperature at 60 ℃ and ultrasonication power was 465 W for 40 min. Under the optimized condition, the yield of total saponin was 16.38%, which is significantly higher than that of traditional heat reflux extraction and ultrasound-assisted extraction methods. In addition,the antioxidant activity of Panax notoginseng saponins extracted by three processes showed that the Panax notoginseng saponins extracted by enzymolysis-ultrasound-assisted surfactant extraction method had higher scavenging ability of DPPH free radicals and stronger total antioxidant capacity. In case of protecting activity against DNA damage,0.156 mg/mL of saponins was optimum. Our finding clearly demonstrated that the developed novel enzymolysis-ultrasound-assisted surfactant extraction method could be applied to gain higher extraction efficiency of saponins, and the developed method is environmentally safe and easy to be scaled up.

Key words： surfactant; ultrasonication; enzymolysis; Panax notoginseng flower; total saponin

Cite this article

CHEN Honghui , TARUN BELWAL , LI Gangfeng , LUO Zisheng . Enzymolysis-ultrasound-assisted surfactant extraction and antioxidant activity of saponins from Panax notoginseng flowers[J]. Food and Fermentation Industries, 2020 , 46(20) : 178 -186 . DOI: 10.13995/j.cnki.11-1802/ts.024843

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