A strain which produce high γ-glutamyltmnspeptidase (GGT) was isolated from an Asian traditional fermented shrimp paste and was identified taxonomically as Bacillus subtilis according to morphological,physiological and biochemical characters and 16S rRNA sequence analysis.The optimal medium and the optimal fermentation condition were determined in this paper:sucrose 25 g/L,tryptone 5 g/L,corn step liquor 15 g/L,MgSO4·7H2O 0.5 g/L and K2HPO4·3H2O 1 g/L ,30 mL medium in 250 mL flask with initial pH 7.2,37 ℃ incubating temperature and shaking cultivation for 16h. Under optimized conditions,the GGT activity could reach 4.07 U/mL. With short-growth cycle and high GGT-producing ability,Bacillus subtilis SK11.004 presented many potential advantages in food industries.