Based on single factor tests,the optimum conditions of lycopene from fresh tomatoes were obtained through Box-benhnken central combination design and RSM. The factors used were power of the microwave extraction (280,490 and 700 W),time of the extraction (120,150 and 180s),and the ratio of solvents to tomato paste (7,8 and 9 mL/g). The optimum extraction conditions were:microwave power 500W,extraction time 160s,ratio of tomato paste to solvents 8.2 mL/g and extraction three times.Under these conditions,lycopene yield was up to 3.047 mg/g.