The fermented rapeseed meal was produced by mixed strains (Laobacillus plantarum R-02 and Bacillus subtilis KGC30141) through solid state anaerobic fermentation. The effects of the inoculation amount of two strains,the adding proportion of corn-steep liquor and glucose,and environmental temperature on the content of glucosinolates and crude protein,the loss of materials in fermented rapeseed meal were researched and optimized by a L18(35)orthogonal experiment. Results show the optimization conditions as follows:the ratio of inoculation quantity of Laobacillus plantarum and Bacillus subtilis was 6%:15%,corn-steep liquor 20%,glucose 3%,environmental temperature 28℃,material to water ratio 1:1,with the 15-day anaerobic fermentation,the glucosinolates content in the fermented rapeseed meal decreased from 84 μmol/g(d.b.) to 33 μmol/g(d.b.),and the crude protein increased by 4.15% compared to original,the loss of materials was 3.73%.