3-HP is a kind of chemical substance that has great perspective,and its biological producing may be much cheaper in cost and much better in security rather than chemical.This study was to isolate microbes producing 3-HP from natural environment,and to try to establish a method of identification of trace metabolite and related microbes.At last 4 strains was gained,which proved the feasibility of the technique we used.The 3-HP yield of these strains were about 0.1%-0.16%(w/v),and all these strains were belong to Klebsiella terrigena.