In this paper,steam-explosion,ultra-fine pulverization,cellulase hydrolysis pre-treatment methods for the brewer's spent grains(BSG)were investigated,and a series of parameters,such as fiber structure,fiber complex,and reducing sugar change,were analyzed.The comparison of fiber structure with pretreatment and without led to the conclusion that the steam-explosion technique can destroy fiber structure,decrease neutral detergent fiber (NDF) content,and increase reducing sugar content.The optimized procedures of cellulase hydrolysis of BSG are as follows:enzyme concentration 180U /g,substrate concentration 10%,temperature 50 ℃,and cellulase hydrolysis time 6h.The increase in content of reducing sugar was amounted to 12.77mg/mL by way of steam-explosion BSG technique and cellulase hydrolysis.However,there was little change in the content of NDF.