A mutant from Aspergillus oryzae which can synthesize β-glucosidase when high concentration glucose exists,was obtained by using agar plate screening techniques,after mutation by UV-60Co.The mutant strain was isolated from the agar plate,which contains 4% glucose.In liquid state fermentation,the mutant strain produced high β-glucosidase activities,which were 17.65IU after 4 days’ culturing.And these were 2.78 times that of the starting strain.The activity of the expressed enzyme was measured by the assay of transglucosidation reaction and the transglucosidation product was identified by TLC and HPLC.At 60% glucose,50℃,pH3.0,and 72 h reaction time,the gentiooligosacharide reached 44.16 g/L.
Zhu Jing
,
Qin Yongling
,
Chen Guiguang
,
Zhang Yunkai
,
Liu Yanjing
,
Luo Jie
,
Liang Zhiqun
. Selection of the High β-glucosidase Mutant and Production of Gentiooligosaccharid[J]. Food and Fermentation Industries, 2010
, 36(04)
: 21
-24,27
.
DOI: 10.13995/j.cnki.11-1802/ts.2010.04.024
