The reliability of three visual identification methods for aflatoxin(AFs)producing strains including yellow-pigment-producing method,ammonia vapor method and ultraviolet fluorescence method were discussed.Beta-cyclodextrin(Mβ-cyd) of low degree substitution could enhance the blue-green fluorescence intensity of AFs producing strain colonies.Fourteen different Asperigillus strains,whose AFs-producing abilities were known,were tested by these methods.Yellow-pigment-producing method on the potato dextrose agar(PDA) was affected by pigment of some strains with error rate 7.1%.Ammonia vapor method was affected by pigment as well with error rate 7.1%.Besides,the error rate of ammonia vapor method was raised to 14.2% because of the color of yeast extract saccharose(YES).AFs-producing strains were distinguished from non-producing ones by ultraviolet fluorescence method without being disturbed by the factors of media color and strain pigment.