Food and Fermentation Industries >

2010 , Vol. 36 >Issue 05: 5 - 8

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.2010.05.018

Food and Fermentation Industries

Study on the Characteristics of Polyphenol Oxidase(PPO) in White Hypsizygus marmoreus

  • Zhou Chun-mei ,
  • Wang Xin ,
  • Wang Jun-cheng ,
  • Liu Bao-lin ,
  • Ren Zhi-chao
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Online published: 2010-05-25

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Abstract

The characteristics of polyphenol oxidase(PPO) in white Hypsizygus marmoreus were studied by spectrophotometer using catechol as the substrate.The results indicated that the absorptive wavelength of enzymatic reactive product was 420nm,and the reaction lasted for at least 3min,while the optimal conditions for PPO were 35℃ and pH 6.5,and the optimal substrate concentration was 0.10 mol/L.The kinetic equation of PPO was 1/V=2.752 264/[S] +17.348 9,and its Km was 0.159 mol/L.The PPO activity of stipe was much higher than other parts.

Key words： white Hypsizygus marmoreus; oolyphenol oxidase; characteristics

Cite this article

Zhou Chun-mei , Wang Xin , Wang Jun-cheng , Liu Bao-lin , Ren Zhi-chao . Study on the Characteristics of Polyphenol Oxidase(PPO) in White Hypsizygus marmoreus[J]. Food and Fermentation Industries, 2010 , 36(05) : 5 -8 . DOI: 10.13995/j.cnki.11-1802/ts.2010.05.018

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