The hydrolysis of waste beer yeast with enzymes such as flavourzyme,papain,neutral protease for preparation of hydrolysis liquid with rich amino acids were investigated.The results showed that hydrolysis efficiency of flavourzyme was better than that of papain or neutral protease comparing with the three proteases.The effects of hydrolysis temperature,time,pH and adding amount of the flavourzyme on yield of amino nitrogen and product were studied,and the optimization conditions were as follows: 10 % beer yeast suspension,pH 5.0,adding flavourzyme 1.6 %,temperature 50 ℃,hydrolysis time 30 h,amino nitrogen 5.9 %.59.7% of product was obtained under the above technical conditions.17 kinds of amino acids were identified using analysis meter of amino acid,and the total concentration of amino acids was 36.89 mg/mL,and the main amino acids were Glu,Ala,Thr,Leu,Arg,Gly,et al.