1,3-1,4-β-glucanases(E.C.3.2.1.73)exhibits a strict substrate specificity for degradation of β-1,4 glycosidic bonds in 3-O-substituted glucopyranose units such as cereal β-glucans and lichenan,which have been widely used in fermentation and feed industry.But its enzyme activity is low under high temperature,it has been a research hotspot to build a 1,3-1,4-β-glucanase with high activity under high temperature.This paper describes the biochemical characteristics of 1,3-1,4-β-glucanase and reviews its gene cloning,expression,thermostability and application research,the research prospects of 1,3-1,4-β-glucanase are also forecasted.