The effects of removing lipid from Lentinus edodes stem at different operation conditions,including extracting time,CO2 pressure,temperature of SFE process were investigated.The results indicated that:(1)The degreased rate of Lentinus edodes was increased rapidly with the increasing of extracting time and it was stable after 50 minutes;(2)The higher temperature the better of degreasing;(3)The most suitable SC-CO2 pressure was(10 MPa).The degreased rate fallen down both above or below this pressure;(4)The rate of recovery of protein-polysaccharides from Lentinus edodes was increased by the degreased rate augment.In summary,SC-CO2 significantly increased protein-polysaccharides extracting from Lentinus edodes(Berk)Sing.