In this research,fifty-nine strains producing fibrinolytic enzyme were successfully isolated from nine kinds of Douchi by using casein in plate at first and secondly by both fibrin plates and plasma plates,and the one DC-YJ11 was the highest fibrinolytic enzyme producing strain.It was identified as Bacillus subtilis by morphological,biochemical,physiological characters analysis,and 16S rDNA sequence analysis.This study also constructed a phylogenetic tree and established its growth model based on BP Neural Network.