Sodium alginate was used as carrier for immobilization of Alcalase.The immobilized conditions of Alcalase and stability of immobilized Alcalase as well as its application in hydrolyzing corn gluten were studied.The results showed that the optimal conditions for immobilizing Alcalase are sodium alginate concentration 3%(w/v),Alcalase concentration 2%(v/v),CaCl2 concentration 2%(w/v).Under these conditions,the optimal conditions for hydrolyzing corn gluten are tertiary butyl alcohol-water system(1∶ 1,v/v),substrate concentration 5%(w/v),dosage of immobilized Alcalase 8%(w/v),pH 8.5,temperature 55℃ and hydrolysis time 2.5h.Under these conditions,the highest hydyolysis degree was 22.7%.After being used for 3 times repeatedly,more than 50% activities of immobilized Alcalase remained.