In this paper,the optimum hydrolytic production process of antioxidant peptide from pine nuts and antioxidant activity was studied.Pine nut protein was used as substrate,based on single-factor tests analysis(enzymolsis pH,enzyme concentration and substrate concentration ratio [E]/[S],enzymolsis temperature,enzymolysis time),using response surface methodology optimized reaction conditions of antioxidant peptides from hydrolysis of Korean pine nut.In addition,the reducing ability and hydroxyl,superoxide radical-scavenging activity and ABTS.scavenging activity of pin nut peptides was also observed.The results showed that optimum conditions of Alcalase 2.4 L enzymatic hydrolysis were determined by response surface methodology as follows pH 8.5,enzyme-substrate ratio 6 250 U/g protein,0.6% substrate concentration,reaction temperature 51 ℃,hydrolysis time 2h.Under the hydrolysis conditions,at 20 mg/mL,the reducing ability of peptides was 84.3% of vitamin C;hydroxyl,superoxide radical-scavenging activity and ABTS.scavenging activity were 96.91%,58.59% and 100%,respectively.