The extraction technology of total flavonoids from bamboo shell was optimized by orthogonal array de-sign.Additionally,antioxidant capacity of the purified total flavonoids were evaluated by determining their.OH and DPPH.and O 2-.scavenging activities.The optimal conditions of extraction were: ethanol concentration 80%,ma-terial/liquid 1∶ 60,temperature 75℃ and extracting time 3 h.Under this conditions the concentration of total fla-vonoids in crude extract was 5.78 mg/g.The purification was performed by macroporous resin HPD600.Both the crude extract and the purified product displayed a good scavenging activity on.OH,DPPH.and O 2-.radical.The IC50 of crude extract and the purified product to.OH,DPPH.and O 2-.radical were 5.25 mg/mL,2.004 mg/mL,948.95 μg/mL and 22.885 4 μg/mL,1.94 mg/mL,1.13 mg/mL.Total flavonoids from bamboo shell is a new kind of effective and natural antioxidant.