Food and Fermentation Industries >

2010 , Vol. 36 >Issue 08: 59 - 63

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.2010.08.007

Food and Fermentation Industries

Purification and Fractionation of Naringinase from Aspergillus niger S-05

  • Tan Xiao-fang ,
  • Hu Jin-rong ,
  • Zhang Jing-sheng ,
  • Zhang Xiu-qing ,
  • Sun Jun-she
Expand

Online published: 2010-08-25

Fold

Abstract

Naringinase from Aspergillus niger S-05 was separated and purified by(NH4) 2SO4 fractional precipita-tion,Sephadex G-25,Cellulose DE-52,Sephadex G-100.The molecular weight estimated by SDS-PAGE.α-L-rham-nosidase has a molecular mass of 66 ku,while β-D-glucosidase has a molecular mass of 102 ku.α-L-rhamnosidase and β-D-glucosidase got 859.21 U/mg and 947.37 U/mg specific activity after purified,with 43.77% and 33.33% yield.

Key words： Aspergillus niger; naringinase; α-L-rhamnosidase; β-D-glucosidase; purification

Cite this article

Tan Xiao-fang , Hu Jin-rong , Zhang Jing-sheng , Zhang Xiu-qing , Sun Jun-she . Purification and Fractionation of Naringinase from Aspergillus niger S-05[J]. Food and Fermentation Industries, 2010 , 36(08) : 59 -63 . DOI: 10.13995/j.cnki.11-1802/ts.2010.08.007

Options
Outlines

/

〈 〉

Powered by Beijing Magtech Co. Ltd,.