The inactivation effect of pulsed electric fields(PEF) on E.coli and S.aureus inoculated into apple juice was investigated.As increasing the electric field strength,total treatment time and electrical conductivity,the inactivation effect of E.coli and S.aureus increased.S.aureus is more sensitive to PEF than E.coli.Both the electric field strength and the total treatment time have a significant inactivation effect on E.coli and S.aureus(P < 0.01).The critical electric field strengths of E.coli and S.aureus are 15.9 kV/cm and 16.4 kV/cm,respectively.Bipolar appears more efficient than monopolar(P < 0.01).The more the energy density input,the better the inactivation effect of PEF on E.coli and S.aureus.