Wheat gluten was hydrolysed by Alcalase 2.4 L FG under the condition of 60℃ and pH 9.0.The ki-netics characteristics of wheat gluten were studied by discussing the influence of different substrate concentration and enzyme concentration on the degree of hydrolysis.Through the experiment,we not only established the kinetic modelsof both hydrolysis rate and degree of hydrolysis,but also discussed the critical substrate concentration and enzyme concentration,and simultaneously calculated the Michaelis constant Km = 1.321 1% coupled with maximum reactionrate Vmax = 0.327 9 mol/(L.h).It was found that the deviation between the theoretical value and actual value wasvery small,which indicated that the model has a good practical application value.Furthermore,the mechanism of controlled enzymatic hydrolysis was probed for a better understanding of intermittent hydrolysis reaction.